Interaction of EL222 with DNA (light 8-min delay)
DOI
https://doi.org/10.71479/p408m-6b985
Record ID
p408m-6b985
Publication date
Jan 5, 2026
Depositors
Gustavo Fuertes affiliationsprincpal contact Orcid link
,
Phuong Ngoc Pham affiliations Orcid link
Associated publication
Article, Front-illuminated surface plasmon resonance biosensor for the study of light-responsive proteins and their interactions
License
CC0 1.0 Universal
Funding references
Time-resolved vibrational spectroscopy of proteins assisted by genetically encoded non-canonical amino acids (Czech Science Foundation (GACR): 24-11819S), Innovation of Czech Infrastructure for Integrative Structural Biology (European Regional Development Fund: CZ.02.01.01/00/23_015/0008175), CIISB, Instruct-CZ Centre of Instruct-ERIC EU consortium (MEYS CR: LM2023042)
Method
Microscale thermophoresis/Temperature related intensity change (MST/TRIC)

Entities of interest

Entity of interest 1

Name
EL222
Type
Polymer
Molecular weight
23157.0 g/mol
Source organism
Erythrobacter litoralis
Sequence
GADDTRVEVQ PPAQWVLDLI EASPIASVVS DPRLADNPLI AINQAFTDLT
GYSEEECVGR NCRFLAGSGT EPWLTDKIRQ GVREHKPVLV EILNYKKDGT
PFRNAVLVAP IYDDDDELLY FLGSQVEVDD DQPNMGMARR ERAAEMLKTL
SPRQLEVTTL VASGLRNKEV AARLGLSEKT VKMHRGLVME KLNLKTSADL
VRIAVEAGIA

Entity of interest 2

Name
dsDNA20
Type
Polymer
Molecular weight
13843.0 g/mol
Sequence
TAG GTA GC C TTT AGT CCA TG
Modifications
Chemical
Type
Atto647N
Position
5'

Results

Result 1

Name
EL222-DNA light 8-min delay
Value
2.0 µM (+ 0.2 - 0.2)
Result type
Half maximal effective concentration EC50

Entity 1

Name
EL222
copy number
2.0

Entity 2

Name
dsDNA20
copy number
1.0

Result 2

Name
EL222-DNA light 8-min delay
Value
1.8 unitless
Result type
Hill coefficient

Entity 1

Name
EL222
copy number
2.0

Entity 2

Name
dsDNA20
copy number
1.0

Chemical environments (buffers)

Name
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%
Constituents
2-(N-morpholino)ethanesulfonic acid 50.0 mM
Sodium chloride 100.0 mM
Pluoronic F-127 0.05 v/v %
pH
6.8

Measurements

Experimental setup

Experiment type
Affinity
Excitation LED
Power: 40.0 Color: RED (ex 605-645nm, em 660-720nm)
Laser power
40.0

Measurement 1

Name
EL222-DNA (rep 1)
Position
1
Targets
dsDNA20 20.0 nM
Ligands
EL222 50000.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 2

Name
EL222-DNA (rep 1)
Position
2
Targets
dsDNA20 20.0 nM
Ligands
EL222 25000.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 3

Name
EL222-DNA (rep 1)
Position
3
Targets
dsDNA20 20.0 nM
Ligands
EL222 12500.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 4

Name
EL222-DNA (rep 1)
Position
4
Targets
dsDNA20 20.0 nM
Ligands
EL222 6250.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 5

Name
EL222-DNA (rep 1)
Position
5
Targets
dsDNA20 20.0 nM
Ligands
EL222 3125.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 6

Name
EL222-DNA (rep 1)
Position
6
Targets
dsDNA20 20.0 nM
Ligands
EL222 1562.5 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 7

Name
EL222-DNA (rep 1)
Position
7
Targets
dsDNA20 20.0 nM
Ligands
EL222 781.3 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 8

Name
EL222-DNA (rep 1)
Position
8
Targets
dsDNA20 20.0 nM
Ligands
EL222 390.6 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 9

Name
EL222-DNA (rep 1)
Position
9
Targets
dsDNA20 20.0 nM
Ligands
EL222 195.3 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 10

Name
EL222-DNA (rep 1)
Position
10
Targets
dsDNA20 20.0 nM
Ligands
EL222 97.7 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 11

Name
EL222-DNA (rep 1)
Position
11
Targets
dsDNA20 20.0 nM
Ligands
EL222 48.8 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 12

Name
EL222-DNA (rep 1)
Position
12
Targets
dsDNA20 20.0 nM
Ligands
EL222 24.4 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 13

Name
EL222-DNA (rep 1)
Position
13
Targets
dsDNA20 20.0 nM
Ligands
EL222 12.2 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 14

Name
EL222-DNA (rep 1)
Position
14
Targets
dsDNA20 20.0 nM
Ligands
EL222 6.1 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 15

Name
EL222-DNA (rep 1)
Position
15
Targets
dsDNA20 20.0 nM
Ligands
EL222 3.1 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 16

Name
EL222-DNA (rep 1)
Position
16
Targets
dsDNA20 20.0 nM
Ligands
EL222 1.5 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 17

Name
EL222-DNA (rep 2)
Position
1
Targets
dsDNA20 20.0 nM
Ligands
EL222 50000.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 18

Name
EL222-DNA (rep 2)
Position
2
Targets
dsDNA20 20.0 nM
Ligands
EL222 25000.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 19

Name
EL222-DNA (rep 2)
Position
3
Targets
dsDNA20 20.0 nM
Ligands
EL222 12500.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 20

Name
EL222-DNA (rep 2)
Position
4
Targets
dsDNA20 20.0 nM
Ligands
EL222 6250.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 21

Name
EL222-DNA (rep 2)
Position
5
Targets
dsDNA20 20.0 nM
Ligands
EL222 3125.0 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 22

Name
EL222-DNA (rep 2)
Position
6
Targets
dsDNA20 20.0 nM
Ligands
EL222 1562.5 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 23

Name
EL222-DNA (rep 2)
Position
7
Targets
dsDNA20 20.0 nM
Ligands
EL222 781.3 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 24

Name
EL222-DNA (rep 2)
Position
8
Targets
dsDNA20 20.0 nM
Ligands
EL222 390.6 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 25

Name
EL222-DNA (rep 2)
Position
9
Targets
dsDNA20 20.0 nM
Ligands
EL222 195.3 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 26

Name
EL222-DNA (rep 2)
Position
10
Targets
dsDNA20 20.0 nM
Ligands
EL222 97.7 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 27

Name
EL222-DNA (rep 2)
Position
11
Targets
dsDNA20 20.0 nM
Ligands
EL222 48.8 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 28

Name
EL222-DNA (rep 2)
Position
12
Targets
dsDNA20 20.0 nM
Ligands
EL222 24.4 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 29

Name
EL222-DNA (rep 2)
Position
13
Targets
dsDNA20 20.0 nM
Ligands
EL222 12.2 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 30

Name
EL222-DNA (rep 2)
Position
14
Targets
dsDNA20 20.0 nM
Ligands
EL222 6.1 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 31

Name
EL222-DNA (rep 2)
Position
15
Targets
dsDNA20 20.0 nM
Ligands
EL222 3.1 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Measurement 32

Name
EL222-DNA (rep 2)
Position
16
Targets
dsDNA20 20.0 nM
Ligands
EL222 1.5 nM
Chemical environment
MES 50 mM NaCl 100 mM Pluronic F-127 0.05%

Instrument

Manufacturer
Nanotemper
Name
Monolith NT.115 RED/GREEN

Files (4)

Download All Files

Raw measurement data

EL222-DNA MST traces (rep 2) Download MST DNA20atto647 EL222_MSTTraceRawData_experiment 6_light_delay 8 min.xlsx

Raw measurement data

EL222-DNA MST traces Download EL222_DNA_LIGHT_8_MIN_DELAY.nta

Raw measurement data

EL222-DNA MST traces (rep 1) Download MST DNA20atto647 EL222_MSTTraceRawData_experiment 3_light_delay 8 min.xlsx

Derived measurement data

EL222-DNA Dose-response + Hill model fit Download MST DNA20atto647 EL222_CompareResultsRawData_Hill model v3_light8mindelay.xlsx