Interaction of hen egg white lysozyme with B09 nanobody
DOI
10.71479/5s81t-w6s49
Record ID
5s81t-w6s49
Publication date
Jan 24, 2025
Depositors
Tatsiana Charnavets* Orcid link
License
CC0 1.0 Universal
Technique
Microscale thermophoresis/Temperature related intensity change (MST/TRIC)

Entities of interest

Entity of interest 1

Name
Lysozyme
Type
Polymer
Molecular Weight
14.3 kDa
Source organism
Gallus gallus

Entity of interest 2

Name
Nanobody B09
Type
Polymer
Molecular Weight
14.2 kDa

Results

Result 1

Name
Kd
Value
1243.0 nM (+ 271.0 - 271.0)
Result type
Constant of dissociation KD

Entity 1

Name
Lysozyme
copy number
1.0

Entity 2

Name
Nanobody B09
copy number
1.0

Chemical environments (buffers)

Name
PBS
Constituents
Sodium chloride 274.0 mM
Potassium chloride 5.4 mM
Disodium hydrogen phosphate 20.0 mM
Potassium dihydrogen phosphate 20.0 mM
pH
7.6

Measurements

Experimental setup

Experiment type
Affinity
Excitation LED
Power: 40.0 Color: RED (ex 605-645nm, em 660-720nm)
Laser power
60.0

Measurement 1

Name
130 uM Lysozyme + 25 nM B09, rep 1
Position
1
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 130.0 µM
Chemical environment
PBS

Measurement 2

Name
130 uM Lysozyme + 25 nM B09, rep 2
Position
1
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 130.0 µM
Chemical environment
PBS

Measurement 3

Name
130 uM Lysozyme + 25 nM B09, rep 3
Position
1
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 130.0 µM
Chemical environment
PBS

Measurement 4

Name
65 uM Lysozyme + 25 nM B09, rep 1
Position
2
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 65.0 µM
Chemical environment
PBS

Measurement 5

Name
65 uM Lysozyme + 25 nM B09, rep 2
Position
2
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 65.0 µM
Chemical environment
PBS

Measurement 6

Name
65 uM Lysozyme + 25 nM B09, rep 3
Position
2
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 65.0 µM
Chemical environment
PBS

Measurement 7

Name
32.5 uM Lysozyme + 25 nM B09, rep 1
Position
3
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 32.5 µM
Chemical environment
PBS

Measurement 8

Name
32.5 uM Lysozyme + 25 nM B09, rep 2
Position
3
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 32.5 µM
Chemical environment
PBS

Measurement 9

Name
32.5 uM Lysozyme + 25 nM B09, rep 3
Position
3
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 32.5 µM
Chemical environment
PBS

Measurement 10

Name
16.25 uM Lysozyme + 25 nM B09, rep 1
Position
4
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 16.25 µM
Chemical environment
PBS

Measurement 11

Name
16.25 uM Lysozyme + 25 nM B09, rep 2
Position
4
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 16.25 µM
Chemical environment
PBS

Measurement 12

Name
16.25 uM Lysozyme + 25 nM B09, rep 3
Position
4
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 16.25 µM
Chemical environment
PBS

Measurement 13

Name
8.125 uM Lysozyme + 25 nM B09, rep 1
Position
5
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 8.125 µM
Chemical environment
PBS

Measurement 14

Name
8.125 uM Lysozyme + 25 nM B09, rep 2
Position
5
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 8.125 µM
Chemical environment
PBS

Measurement 15

Name
8.125 uM Lysozyme + 25 nM B09, rep 3
Position
5
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 8.125 µM
Chemical environment
PBS

Measurement 16

Name
4.0625 uM Lysozyme + 25 nM B09, rep 1
Position
6
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 4.0625 µM
Chemical environment
PBS

Measurement 17

Name
4.0625 uM Lysozyme + 25 nM B09, rep 2
Position
6
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 4.0625 µM
Chemical environment
PBS

Measurement 18

Name
4.0625 uM Lysozyme + 25 nM B09, rep 3
Position
6
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 4.0625 µM
Chemical environment
PBS

Measurement 19

Name
2.03125 uM Lysozyme + 25 nM B09, rep 1
Position
7
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 2.03125 µM
Chemical environment
PBS

Measurement 20

Name
2.03125 uM Lysozyme + 25 nM B09, rep 2
Position
7
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 2.03125 µM
Chemical environment
PBS

Measurement 21

Name
2.03125 uM Lysozyme + 25 nM B09, rep 3
Position
7
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 2.03125 µM
Chemical environment
PBS

Measurement 22

Name
1.015625 uM Lysozyme + 25 nM B09, rep 1
Position
8
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 1.015625 µM
Chemical environment
PBS

Measurement 23

Name
1.015625 uM Lysozyme + 25 nM B09, rep 2
Position
8
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 1.015625 µM
Chemical environment
PBS

Measurement 24

Name
1.015625 uM Lysozyme + 25 nM B09, rep 3
Position
8
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 1.015625 µM
Chemical environment
PBS

Measurement 25

Name
0.5078125 uM Lysozyme + 25 nM B09, rep 1
Position
9
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.5078125 µM
Chemical environment
PBS

Measurement 26

Name
0.5078125 uM Lysozyme + 25 nM B09, rep 2
Position
9
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.5078125 µM
Chemical environment
PBS

Measurement 27

Name
0.5078125 uM Lysozyme + 25 nM B09, rep 3
Position
9
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.5078125 µM
Chemical environment
PBS

Measurement 28

Name
0.2539063 uM Lysozyme + 25 nM B09, rep 1
Position
10
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.2539063 µM
Chemical environment
PBS

Measurement 29

Name
0.2539063 uM Lysozyme + 25 nM B09, rep 2
Position
10
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.2539063 µM
Chemical environment
PBS

Measurement 30

Name
0.2539063 uM Lysozyme + 25 nM B09, rep 3
Position
10
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.2539063 µM
Chemical environment
PBS

Measurement 31

Name
0.1269531 uM Lysozyme + 25 nM B09, rep 1
Position
11
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.1269531 µM
Chemical environment
PBS

Measurement 32

Name
0.1269531 uM Lysozyme + 25 nM B09, rep 2
Position
11
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.1269531 µM
Chemical environment
PBS

Measurement 33

Name
0.1269531 uM Lysozyme + 25 nM B09, rep 3
Position
11
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.1269531 µM
Chemical environment
PBS

Measurement 34

Name
0.0634766 uM Lysozyme + 25 nM B09, rep 1
Position
12
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0634766 µM
Chemical environment
PBS

Measurement 35

Name
0.0634766 uM Lysozyme + 25 nM B09, rep 2
Position
12
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0634766 µM
Chemical environment
PBS

Measurement 36

Name
0.0634766 uM Lysozyme + 25 nM B09, rep 3
Position
12
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0634766 µM
Chemical environment
PBS

Measurement 37

Name
0.0317383 uM Lysozyme + 25 nM B09, rep 1
Position
13
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0317383 µM
Chemical environment
PBS

Measurement 38

Name
0.0317383 uM Lysozyme + 25 nM B09, rep 2
Position
13
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0317383 µM
Chemical environment
PBS

Measurement 39

Name
0.0317383 uM Lysozyme + 25 nM B09 rep 3
Position
13
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0317383 µM
Chemical environment
PBS

Measurement 40

Name
0.0158691 uM Lysozyme + 25 nM B09 rep 1
Position
14
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0158691 µM
Chemical environment
PBS

Measurement 41

Name
0.0158691 uM Lysozyme + 25 nM B09, rep 2
Position
14
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0158691 µM
Chemical environment
PBS

Measurement 42

Name
0.0158691 uM Lysozyme + 25 nM B09, rep 3
Position
14
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0158691 µM
Chemical environment
PBS

Measurement 43

Name
0.0079346 uM Lysozyme + 25 nM B09, rep 1
Position
15
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0079346 µM
Chemical environment
PBS

Measurement 44

Name
0.0079346 uM Lysozyme + 25 nM B09, rep 2
Position
15
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0079346 µM
Chemical environment
PBS

Measurement 45

Name
0.0079346 uM Lysozyme + 25 nM B09, rep 3
Position
15
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0079346 µM
Chemical environment
PBS

Measurement 46

Name
0.0039673 uM Lysozyme + 25 nM B09, rep 1
Position
16
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0039673 µM
Chemical environment
PBS

Measurement 47

Name
0.0039673 uM Lysozyme + 25 nM B09, rep 2
Position
16
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0039673 µM
Chemical environment
PBS

Measurement 48

Name
0.0039673 uM Lysozyme + 25 nM B09, rep 3
Position
16
Targets
Nanobody B09 25.0 nM
Ligands
Lysozyme 0.0039673 µM
Chemical environment
PBS

Instrument

Manufacturer
Nanotemper
Name
Monolith NT.115 RED/GREEN

Files

Raw measurement data Download Lys VHH B09 25 nM Lys100uM max_MSTTraceRawData.xlsx Derived measurement data Download Lys VHH B09 25 nM Lys100uM max_CompareResultsRawData.xlsx