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"plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "E5" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "38a5ebfa-fc8e-4518-9da2-0dda486099ec", "measurement_protocol_step": { "id": "54c9954c-3fc4-4b18-b20f-c84143eda83a", "name": "Third baseline (immediately after illumination)" }, "name": "Set 2: EL222 6 uM third baseline", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 6.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "H8" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "93a61143-a7db-4669-87b8-10ce31a3f3e8", "measurement_protocol_step": { "id": "54c9954c-3fc4-4b18-b20f-c84143eda83a", "name": "Third baseline (immediately after illumination)" }, "name": "Set 2: EL222 4 uM third baseline", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 4.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "B5" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "2d217fef-f7ca-4929-bba3-391fa27f6bdb", "measurement_protocol_step": { "id": "54c9954c-3fc4-4b18-b20f-c84143eda83a", "name": "Third baseline (immediately after illumination)" }, "name": "Set 2: EL222 2 uM third baseline", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 2.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "F5" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "682a1de7-acc0-4183-9c78-315993d99f9f", "measurement_protocol_step": { "id": "54c9954c-3fc4-4b18-b20f-c84143eda83a", "name": "Third baseline (immediately after illumination)" }, "name": "Set 2: EL222 0 uM association (third baseline)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 0.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": 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5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "D6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "5930834c-d2d4-4c2f-b968-cbe54a9bf27b", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 16 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 16.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "C6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "a67ecd51-405f-4bf1-a113-fe4dbf595887", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 12 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 12.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "G9" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "3122480c-e4cd-451f-aeb9-9c7f7d9c5216", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 8 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 8.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "E6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "c6738b80-40fa-435d-a0cd-9fe1ed1d24d6", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 6 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 6.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "H9" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "9251d9e8-f9f5-4cd1-bcf1-bb97648f3b2d", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 4 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 4.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "B6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "3042aba0-dc14-4f2c-ba39-cfb2e0f415df", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 2 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 2.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "preparation_protocol": [ { "description": "Samples were illuminated ex situ for 300 seconds with a wavelength of 450 nm and an irradiance of around 5 mW/cm2. The time delay between the end of the illumination and the start of the association phase was 3 minutes (due to the 3 minutes requrired to measure the third baseline)", "name": "Ex situ illumination" } ], "well_position": "F6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "81ca0f61-80ef-4ef9-94cc-e4445310a799", "measurement_protocol_step": { "id": "fd471101-1865-4b37-8c4d-41973305464b", "name": "EL222-DNA association (3 minutes after illumination)" }, "name": "Set 2: EL222 0 uM association (3 minutes after illumination)", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 0.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "G6" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "f3e03646-46b8-455b-84bc-344bf0dfb756", "measurement_protocol_step": { "id": "372a4d87-0b6b-4adb-bcfd-b216ea1810c1", "name": "EL222-DNA dissociation (8 minutes after illumination)" }, "name": "Set 2: EL222 20 uM dissociation", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 20.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "D7" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "732e01f6-4588-4a90-9043-8358549dc83e", "measurement_protocol_step": { "id": "372a4d87-0b6b-4adb-bcfd-b216ea1810c1", "name": "EL222-DNA dissociation (8 minutes 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"id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "G10" }, "sensor": { "id": "2153b699-b73f-417d-a1f7-9a857f4c0e86", "name": "Streptavidin (SA)" } }, { "id": "d265e8e6-b687-41c6-8019-12c8fce86375", "measurement_protocol_step": { "id": "372a4d87-0b6b-4adb-bcfd-b216ea1810c1", "name": "EL222-DNA dissociation (8 minutes after illumination)" }, "name": "Set 2: EL222 8 uM dissociation", "sample": { "analytes": [ { "concentration": { "unit": "\u00b5M", "value": 8.0 }, "entity": { "id": "48ea2c66-1da9-4fd7-9c80-03c5f86944b1", "name": "EL222" } } ], "chemical_environment": { "id": "acb18049-a83b-4d44-9577-e7c44e7a61ec", "name": "MES 50 mM, NaCl 150 mM, BSA 250 \u00b5g/mL" }, "plate": { "id": "6e8f7cb1-24a7-4b42-a51d-403b0724478c", "name": "96-well sample plate 1 (p1)" }, "well_position": "E7" }, "sensor": { "id": 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